标题：Termination of transcription by Escherichia coli RNA polymerase: Influence of secondary structure of RNA transcripts on ρ-independent and ρ-dependent termination
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1979
卷号：76
期号：4
页码：1613-1617
DOI：10.1073/pnas.76.4.1613
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The effect of RNA secondary structure on {rho}-independent and {rho}-dependent termination of transcription of T3 DNA by Escherichia coli RNA polymerase has been studied by incorporating, into nascent transcripts, base analogs that lead to altered base-pairing properties. A guanine [->] hypoxanthine substitution, with attendant weakening of secondary structure, abolished the {rho}-independent termination at 20% of the genome; in contrast, replacement of cytosine with 5-bromocytosine, which forms stronger pairs with guanine, enhanced termination at this site. {rho}-Independent termination was not altered by replacing uracil with 5-bromouracil. There are two major {rho}-dependent termination sites on the T3 DNA--at 8 and 15%. The termination activity of {rho} in this system also depended on RNA secondary structure. The incorporation of 5-bromouracil instead of uracil into RNA did not alter the site specificity of {rho} action but {rho} was rendered inactive when cytosine was replaced by 5-bromocytosine. In contrast, replacement of GTP with ITP in the reaction increased {rho}-dependent inhibition of RNA synthesis, caused production of heterogeneous-sized transcripts, and stimulated {rho}-mediated ATP hydrolysis. The {rho}-associated ATPase activity, in the presence of isolated T3 RNA, was also stimulated by inosine substitution. Furthermore, the temperature-sensitive {rho} isolated from rho 15 mutant of E. coli, which does not terminate transcription in the presence of the common rNTPs, was active when GTP was replaced with ITP. These results suggest that strongly paired G{middle dot}C-rich regions in RNA stem-loop structures or RNA{middle dot}DNA hybrids are essential for {rho}-independent termination, whereas {rho}-dependent termination requires weakly paired cytosine residues for its action.
