期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1979
卷号：76
期号：4
页码：1972-1976
DOI：10.1073/pnas.76.4.1972
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Purified human peripheral blood T lymphocytes were fractionated by density gradient electrophoresis on the basis of their surface charge. The high mobility cell fractions were found to be enriched in T cells having receptors for IgM (T{micro} cells) with only minor proportions of T cells having receptors for IgG (T{gamma} cells). In contrast, the low mobility cell fractions were enriched in T{gamma} cells with very low numbers of contaminating T{micro} cells. Both populations were of higher mobility than human peripheral blood B lymphocytes. High-affinity sheep erythrocyte resette-forming cells (E-RFC) were relatively enriched in the high and intermediate mobility fractions and appear to include the T{micro} cells and the T cells without receptors for IgG or IgM (T{phi}). The low affinity E-RFC were found only in the lower mobility fractions that included the T{gamma} cell population. A direct correlation was observed between the number of T{gamma} lymphocytes and the low affinity E-RFC in all the fractions. The separated cell fractions were treated in vitro with different concentrations of concanavalin A (Con A) and examined for the numbers of T{micro} and T{gamma} cells. Low concentrations of Con A (2.5 {micro}g/ml) significantly increased the number of T{micro} cells, whereas high concentrations of Con A (20 {micro}g/ml and 40 {micro}g/ml) markedly reduced the number of T{micro} cells and increased the number of T{gamma} cells. Furthermore, all fractions (both T{micro} and T{gamma} cell enriched) responded by proliferation to Con A, whereas only the high and intermediate mobility fractions (enriched in T{micro} cells) responded to phytohemagglutinin. Fractions enriched in T{gamma} cells responded very poorly to phytohemagglutinin. This method provides another technique for separating human T{micro}- and T{gamma}-enriched lymphocyte subpopulations and does not modulate the Fc receptors of the cells, in contrast to the rosetting techniques currently in use for the separation of these lymphocytes.
