期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1979
卷号：76
期号：8
页码：4033-4037
DOI：10.1073/pnas.76.8.4033
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：T cells were isolated from spleens of C57BL/Ka/Thy 1.1/Lb mice immunized with 2,4-dinitrophenylated keyhole limpet hemocyanin (DNP-KLH). The mice were infected in vitro with radiation leukemia virus and injected intrathymically into congenic C57BL/Ka (Thy 1.2) recipients. Within 3-4 months, seven thymic lymphomas developed, five of which were of donor type. The donor lymphomas were explanted and permanent cell lines were established in vitro. These lymphoma cells are capable of providing antigen-specific carrier (KLH)-primed help to DNP-primed B lymphocytes in secondary antibody production to DNP-KLH. They also enhance the secondary antibody response of whole period spleen cell populations to DNP-KLH. The availability of these immortal clonable populations of immunofunctional neoplastic T lymphocytes should facilitate biological and biochemical investigations of lymphocyte interactions during synthesis of antibody to thymus-dependent antigens.
