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  • 标题:Molecular cloning of a human histocompatibility antigen cDNA fragment
  • 本地全文:下载
  • 作者:H L Ploegh ; H T Orr ; J L Strominger
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1980
  • 卷号:77
  • 期号:10
  • 页码:6081-6085
  • DOI:10.1073/pnas.77.10.6081
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.
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