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  • 标题:The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region
  • 本地全文:下载
  • 作者:H Garoff ; A M Frischauf ; K Simons
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1980
  • 卷号:77
  • 期号:11
  • 页码:6376-6380
  • DOI:10.1073/pnas.77.11.6376
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The amino acid sequence of the capsid (C) protein was deduced from the nucleotide sequence of the C gene. This part of the viral 42S RNA genome was transcribed into double-stranded cDNA. The cDNA was cloned in the Escherichia coli chi 1776-pBR322 host-vector system and then the base sequence was determined with the technique described by Maxam and Gilbert. The amino acid sequence of the C protein shows a clustering of basic amino acids and prolines within the first 110 amino acids.
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