期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：11
页码：6639-6643
DOI：10.1073/pnas.77.11.6639
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Tiny fragments from the cytoplasm of human skin fibroblasts with about 2% of the original cell volume ("microplasts") were prepared by treatment with cytochalasin B, vigorous pipetting, and trypsinization of the attached fragments. They remained alive for 8 hr or longer. Some of the microplasts were able to produce and move filopodia, ruffle, or both; others blebbed continuously. Slow flattening was observed in the larger microplasts. In all cases tested, microplasts avoided contact with other cells or microplasts. The observations suggest that the cytoplasmic matrix and the membranes of animal cells are so constructed as to express locally and autonomously any one of the elementary amoeboid movements listed above. More importantly, whatever types of motile surface projections a microplast expressed, it continued to produce and move them in a stereotypical way as if there were long-lived structural or material determinants for each type. The microplasts were unable to locomote autonomously. Therefore, it is conceivable that directional movement of whole cells may require a supervising mechanism that confers a certain coordination and strategy on its component cytoplasmic bits. Otherwise they would continue to move in stereotypical and autonomous ways without ever displacing themselves, as suggested by the behavior of the microplasts.
