期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：2
页码：804-807
DOI：10.1073/pnas.77.2.804
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Transfer of phospholipid from the envelope of Sendai virus to erythrocyte membrane was measured by using spin-labeled phosphatidylcholine. The transfer was enhanced autocatalytically above a threshold dose (about five adsorbed viruses per cell). There was an inflection point in the time course of transfer, after which the transfer was greatly accelerated. The time to reach the inflection point became shorter with increased viral dose. The transfer reaction was markedly enhanced above 19 degrees C and the inflection point was observed above this temperature. There was negligible transfer from trypsin-treated virus to erythrocyte membrane, but the transfer was greatly enhanced by intact virus. The enhancement was larger with increased amount of intact virus, and the inflection point was observed in the transfer curves. All the kinetic data can be satisfactorily analyzed by a model which assumes that the virus modifies the cell membrane at the attachment site, the modification is propagated in the membrane, and transfer of phospholipid to the modified sites is greatly enhanced. The propagation rate is estimated as approximately equal to 10(-11) cm2s-1 and the activation energy as 13 kcal mol-1 (54 kJ mol-1). The viral F glycoproteins are suggested as a possible entity for the modification and its propagation: they are introduced into the target cell membrane by envelope fusion, diffuse laterally, and enhance both phospholipid exchange and envelope fusion with viruses attached to the membrane sites.
