期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1980
卷号:77
期号:4
页码:1937-1941
DOI:10.1073/pnas.77.4.1937
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:The nuclear RNA from a large variety of kappa-producing plasmacytomas was size fractionated and analyzed with a series of cloned probes representing sequences encoding variable (V), joining (J), and constant (C) regions and selected intervening sequences. All of the plasmacytomas produce a nuclear RNA component that contains V kappa and C kappa sequences as well as the intervening sequence between J kappa and C kappa, and that has a distinctive size depending on which of the four J kappa segments is expressed (i.e., is present in the secreted kappa chain). These RNAs are the precursors of kappa mRNAs, which are transcribed from productively rearranged C kappa genes. Half of the plasmacytomas examined produce, in addition to a kappa mRNA precursor, a discrete component of about 8.4 kilobases that contains C kappa and upstream flanking sequences but lacks the expressed V region sequence. The ability to produce this component is always associated with the persistence in the tumor genome of an unrearranged (germline) J kappa-C kappa region. In tumors rearranged at both kappa loci the nonproductive allele is either transcriptionally silent or, in a minority of cases, transcribed and processed into a "fragment" mRNA lacking V region sequences. These results reveal that allelic exclusion can be effected at several levels of gene expression. They also provide some insight into the relative contributions of the V and C gene elements to this expression.