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  • 标题:Genes for three Drosophila heat-shock-induced proteins at a single locus
  • 本地全文:下载
  • 作者:S C Wadsworth ; E A Craig ; B J McCarthy
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1980
  • 卷号:77
  • 期号:4
  • 页码:2134-2137
  • DOI:10.1073/pnas.77.4.2134
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Two plasmids containing cDNA inserts complementary to the messenger RNAs for the 26,000 and 23,000 molecular weight heat-shock-induced proteins from Drosophila melanogaster were identified. Both of these probes hybridized to a site of a major heat-shock-induced puff, 67B. Hybridization by the gel transfer technique with genome DNA that had been cleaved by a restriction endonuclease indicated that the two genes are closely linked. This expectation was validated through isolation of a DNA fragment generated by BamHI endonuclease containing genes for the 26,000 and 23,000 molecular weight heat-shock-induced proteins and also a gene encoding the 27,000 molecular weight heat-shock-induced protein.
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