期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：4
页码：2153-2157
DOI：10.1073/pnas.77.4.2153
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：If lambda DNA replication is blocked by mutation in any one of several genes essential for replication, intracellular lambda DNA often shows short three-stranded regions called D loops. In this report we show that one arm of a D loop is an RNA . DNA hybrid, whereas the remaining arm is made up of single-stranded DNA. The RNA can be partially removed by RNase A and totally removed by RNase H. Also, D loops do not appear if infections are made in cells treated with rifampin, a potent inhibitor of transcription by Escherichia coli RNA polymerase. Several genes associated with recombination, including the host recA gene, are not essential for D-loop formation.
