期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：5
页码：2555-2558
DOI：10.1073/pnas.77.5.2555
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cell-free extracts of nifF and nifJ mutants of Klebsiella pneumoniae are unable to couple acetylene reduction (N2 fixation) by nitrogenase to the oxidation of organic metabolites. However, nifF and nifJ mutants can complement each other in vitro to establish the coupling. This indicates that the products of the nifF and nifJ genes constitute essential elements of the physiological electron pathway to nitrogenase. The electron-transfer-active product of the nifF gene, a flavoprotein, has been purified.
