期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：5
页码：2772-2776
DOI：10.1073/pnas.77.5.2772
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The location of calmodulin in rabbit and guinea pig spermatozoa was determined by indirect immunofluorescence techniques. Spermatozoa that had not undergone the acrosome reaction exhibited four distinct regions of calmodulin-specific immunofluorescence: around the acrosome, in a band across the lower third of the head, and in two localized areas at the base and tip of the flagellum. In contrast, after the acrosome reaction, although other features of calmodulin distribution remained the same, the fluorescence associated with the anterior half of the head was notably absent. Instead, fluorescence was associated with the membranes that had separated from the sperm head. These findings suggest a potential role for calmodulin in the Ca2+-dependent control of sperm activation, in sperm-egg fusion, and in microtubule disassembly processes in the flagellum.
