期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：5
页码：2899-2903
DOI：10.1073/pnas.77.5.2899
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Standard cell-fusion techniques have been used to generate hybrid cells from rabbit spleen cells and mouse myeloma cell lines. The hybrids were selected for secretion of rabbit immunoglobulin. Detailed allotype analyses were carried out for 189 cell lines found to be immunoglobulin positive: 1 produced an intact immunoglobulin molecule with antibody activity, 143 produced rabbit light (L) chains, 36 produced rabbit heavy (H) chains, and 9 cell lines gave negative results in tests for group a and b allotypes. Fusions with a nonproducing murine myeloma cell line (SP-2) yielded only L chain-secreting hybrids, whereas 27% of hybrids resulting from fusion with an L chain-producing line (PU) secreted rabbit H chains paired with mouse L chains. Several of the hybridomas have maintained the ability to produce rabbit immunoglobulin chains in culture for almost 1 year and can be propagated as ascites tumors in athymic (nude) mice. Cytogenetic analyses have detected no rabbit chromosomes in the stable hybrid cell lines.
