期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：6
页码：3590-3594
DOI：10.1073/pnas.77.6.3590
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structural genes for Escherichia coli ribosomal protein (r-protein) genes L1, S4, and S11 were inserted into a plasmid vector containing the lac operator and promoter such that the synthesis of L1, S4, and S11 was controlled by lac regulatory elements. Synthesis of L1, S4, and S11 was stimulated by addition of an inducer of the lac operon (isopropyl thiogalactoside) to exponentially growing cells. Elevated synthesis of L1 caused a specific decrease in L11 synthesis, whereas overproduction of S4 resulted in lowered synthesis of S13 and L17. Stimulation of L1 or S4 synthesis also inhibited cell growth. Overproduction of S11 did not affect synthesis of other r-proteins or alter growth. These results confirm previous in vitro studies [Yates, J. L., Arfsten, A. E. & Nomura, M. (1980) Proc. Natl. Acad. Sci. USA 77, 1837-1841] and support the hypothesis that certain r-proteins have the capacity to selectively inhibit synthesis of r-proteins whose genes are in the same operon as their own.
