期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：6
页码：3625-3629
DOI：10.1073/pnas.77.6.3625
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In a study designed to determine whether T cells of man and higher primates express a surface component related to the variable region of immunoglobulin heavy chain (VH), chickens were immunized with the purified VH fragment of a monoclonal Waldenstrom macroglobulin. The antibody preparation reacted with a mu chain determinant contained in the Fd fragment and with individual determinants characteristic of the orginal Waldenstrom protein. As estimated by immunofluorescence analysis, a subpopulation of normal human peripheral T cells (approximately 30%) bound the anti-VH antibody. B-Cell lymphoma lines grown in vitro, as well as some T-cell leukemia lines of the cotton-topped marmoset (Sagiunus oedipus), also bound the anti-VH antibody. The VH-bearing component of the T-cell line 70-N-2 was labeled biosynthetically by incorporation of [3H]leucine and was precipitated specifically by anti-VH antibody. This component was characterized by an apparent mass of 70,000 daltons as assessed by polyacrylamide gel electrophoresis under reducing conditions in buffers containing sodium dodecyl sulfate. These data provide direct support for the hypothesis that some T cells express and synthesize a component related to immunoglobulin heavy chains.
