期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：6
页码：3664-3668
DOI：10.1073/pnas.77.6.3664
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have studied the events that occur during the development of chemotaxis in HL60, a promyelocytic leukemia cell line that acquires the features of mature neutrophils when exposed to dimethylformamide (DMF). Chemotactic function first appears between 48 and 96 hr of DMF induction and is associated not only with the coincidental development of deformability, spontaneous motility, greatly increased binding of fMet-Leu-Phe, and orientation but also with decreasing cell size and pleomorphism of nuclei. Surface adhesiveness develops earlier (36-48 hr) and is coincident with a 10-fold increase in protein synthesis not seen in other DMF-inducible cell lines. This burst of protein synthesis precedes the expression of chemotactic function. These studies show that the HL60 cell line can provide a useful model for delineating control mechanisms responsible for the development of complex cellular functions present in differentiated myeloid cells in humans.
