期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：8
页码：4430-4434
DOI：10.1073/pnas.77.8.4430
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The presence of particular surface carbohydrate modifications is shown to affect dramatically the stability and tissue specificity of unilamellar distearoyl phosphatidylcholine vesicles in mice. Use of the gamma-ray probe 111In3+ permits analysis of tissue distributions by standard gamma counting techniques and determination of the structural integrity of the vesicles by perturbed angular correlation spectroscopy. Addition of a 6-aminomannose derivative of cholesterol to the lipid bilayer produces initial retention of high levels of intact vesicles in the lung after intravenous injection followed by concentration of intact vesicles in the liver and spleen. Vesicles bearing 6-aminosugar residues are found to concentrate in the axillary space in aggregates of polymorphonuclear leukocytes when administered subcutaneously. The in vivo stability of 6-aminomannose-labeled vesicles is substantially greater after intravenous or subcutaneous administration than that observed for any other system examined. The dose-response effects observed with surface modifications indicate that a particular receptor topography is important in the mechanism leading to transport and destruction of these vesicles.
