期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：9
页码：5268-5272
DOI：10.1073/pnas.77.9.5268
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The addition of 10% dimethyl sulfoxide (Me2SO) to PtK2 and WI-38 cells caused stress fibers to disappear from the cytoplasm and numerous elongated inclusions to appear in the nucleus. When Me2SO was removed, the stress fibers reformed and the nuclear inclusions disappeared. These nuclear inclusions reacted with fluorescent heavy meromyosin, phalloidin, and actin antibody. In the electron microscope, needle-like structures were seen to be composed of wavy filaments that bound heavy meromyosin. Antibodies against other components of stress fibers--tropomyosin, alpha-actinin, and myosin--did not react with the inclusions. When fluorescently labeled actin was microinjected into living PtK2 and WI-38 cells, the fluorescent actin was incorporated into stress fibers. Subsequent exposure of the same cells to Me2SO led to breakdown of the fluorescent stress fibers and the appearance of fluorescent inclusions in the nucleus. Removal of Me2SO caused reversion to the normal interphase structure. These results indicate that under the influence of Me2SO, dissolution of stress fiber releases actin in a form which allows it to diffuse into the nucleus where it then becomes organized into filamentous bundles.
