期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：1
页码：564-568
DOI：10.1073/pnas.78.1.564
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A cloned cell line derived from mouse bone marrow and transformed by Abelson virus is shown to synthesize two different heavy chains, mu and gamma 2B, in vitro. This characteristic is stable because it persists upon subcloning. Although most of the immunoglobulin-synthesizing cells produce either mu or gamma 2B heavy chains, a few cells contain both heavy chains, suggesting immunoglobulin class switching. Karyotypes show a complement of 41 chromosomes. Two copies of chromosome 12, to which immunoglobulin heavy chain structural genes have been assigned, were found. No light chain was found in either the mu- or the gamma 2B-producing cells. However, fusion of the cell line with a myeloma that synthesizes neither heavy nor light chains caused expression of kappa light chain in the hybridoma synthesizing mu chain. No light chain could be detected in the hybridomas synthesizing gamma 2B heavy chain.
