期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：1
页码：544-548
DOI：10.1073/pnas.78.1.544
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In prior studies a heteroantiserum to a surface membrane component termed TH2 was used to define two subsets of human T cells (TH2+ and TH2-), which were found to express distinct sets of activities in vitro. In the present studies we prepared monoclonal antibodies to surface determinants that are restricted to T cells belonging to each of these two subsets. Two antibodies, termed Leu-2a and Leu-2b, which seem to define the same surface antigen identified by the original TH2 antiserum, reacted with 57-84% of thymocytes and 22-46% of the erythrocyte-rosette-forming cells (ERF-C) in peripheral blood. Two other monoclonal antibodies, termed Leu-3a and Leu-3b, reacted with the same subpopulation of thymocytes (78-89%) and peripheral blood ERF-C (47-78%) but, unlike Leu-2a and Leu-2b, did not exhibit cross-blocking; i.e., labeling cells with Leu-3a did not inhibit the subsequent binding of Leu-3b. T cells reactive with Leu-2a were shown to be unreactive with Leu-3a, indicating that two separate subpopulations of T cells, Leu-2 (formerly TH2+) and Leu-3 (TH2-) T cells, were thereby defined. These two T cell subsets make up the subpopulation of ERF-C (80-95%) previously defined by a monoclonal antibody to a T cell membrane antigen (Leu-1) that has a thymus-dependent distribution on normal lymphocytes but is expressed by some surface-immunoglobulin-positive (sIg+) leukemic lymphocytes. None of the Leu antibodies reported here reacted with sIg+, Leu-1+ leukemic cells, nor did they react with normal hematopoietic cells or lymphoid cells that had surface markers characteristic of B cells. Studies of the blocking effects of Leu antibodies on killing in cell-mediated lympholysis by effector T cells were carried out in the absence of complement. These experiments established the following points: (i) Leu-2a abolished the killing by cytotoxic T cells of allogeneic phytohemagglutinin-stimulated blasts, (ii) inhibition of killing by Leu-2b was markedly less than inhibition by Leu-2a, and (iii) other antibodies, including Leu-1, Leu-3a, and Leu-3b, had little or no effect on killing in cell-mediated lympholysis. The relevance of these findings to prior studies done in the mouse and in man are discussed.
