期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：10
页码：6226-6230
DOI：10.1073/pnas.78.10.6226
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Analysis of the metastatic properties of clones isolated from mouse B16 melanoma cell lines (B16-F1 and F10) shows extensive cellular heterogeneity and the presence of subpopulations that have widely differing metastatic abilities. This pattern of metastatic heterogeneity is maintained during serial passage in vitro and in vivo. In contrast, even a short serial passage of individual clones isolated from these heterogeneous parent lines results in rapid emergence of variant subclones that have different metastatic properties. If several clones are mixed and cocultivated, this instability is not expressed. These data suggest that, in polyclonal populations, the various clonal subpopulations somehow interact with one another to "stabilize" their relative proportions within the population. Restriction of clonal diversity by selective killing of the majority of clones in a polyclonal population eliminates the stabilizing restraints and stimulates rapid emergence of new subpopulations to create heterogeneous populations containing a new panel of phenotypically diverse subpopulations that then reach stable proportions until the next selection pressure(s) is encountered.
