期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：11
页码：6579-6583
DOI：10.1073/pnas.78.11.6579
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The four stereoisomers of 25-hydroxyvitamin D3 26,23-lactone were synthesized by a stereoselective lactonization method. Natural 25-hydroxyvitamin D3 26,23-lactone was produced from 25-hydroxy-[3-3H]vitamin D3 by in vitro incubation of kidney homogenate prepared from vitamin D-supplemented chickens or was isolated from the serum of rats given 1,25-dihydroxyvitamin D3 and 25-hydroxy-[3-3H]vitamin D3. The four synthetic isomers and the naturally produced 25-hydroxyvitamin D3 26,23-lactone were subjected to high-performance liquid chromatography in a system capable of separating the four isomers. The natural lactone comigrated with synthetic (23S,25R)-25-hydroxyvitamin D3 26,23-lactone, establishing it as the natural vitamin D3 metabolite.
关键词：vitamin D metabolite ; iodolactonization ; phenylselenolactonization
