标题：Methylmercury(II) binding to single-stranded and duplex DNA: complexes formed are distinguishable by optical detection of magnetic resonance spectroscopy
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：5
页码：2972-2976
DOI：10.1073/pnas.78.5.2972
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Binding of CH3Hg(II) to duplex and single-stranded calf thymus DNA leads to an external heavy atom effect that is associated with the formation of complexes directly with the purine and pyrimidine bases. When CH3Hg(II) is added at a concentration insufficient to cause denaturation, clearly distinguishable optical detection of magnetic resonance spectra are observed from the duplex and single-stranded DNA complexes. Comparison of the dominant signals with those observed from CH3Hg(II) complexes of model mononucleotides and mononucleosides allows their identification as guanine complexed at N7 in the duplex sample and thymine complexed at N3 in the single-stranded sample. On the basis of these measurements, it is estimated that this experiment presently is capable of detecting about 2% single-stranded DNA in a sample made up predominantly after duplex structure.
