标题：Adrenocorticotropin-like and alpha-melanotropin-like peptides in a subpopulation of human gastrin cell granules: bioassay, immunoassay, and immunocytochemical evidence
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：5
页码：2990-2994
DOI：10.1073/pnas.78.5.2990
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Adrenocorticotropin (ACTH)-like and alpha-melanotropin (alpha-MSH)-like peptides have been localized to a subpopulation of cytoplasmic (secretory) granules of human antropyloric gastric cells and of fetal and neoplastic gastrin cells. These granules also store gastrin and belong to the electron-dense variety of gastrin cell granules. Gastrin cells also contain granules of low to medium electron density; these store only gastrin and do not react with ACTH or alpha-MSH antisera. The alpha-MSH immunoreactive peptide was shown also to display alpha-MSH bioactivity by a combined immunosorbent-bioassay technique. This peptide cochromatographs with synthetic alpha-MSH in several systems and is not detected in oxyntic mucosa or in gastric muscle wall. As in the pituitary intermediate lobe, the alpha-MSH-like peptide may be formed by cleavage of ACTH-like peptides also in gastrin cells. These data provide additional evidence for local formation of ACTH/alpha-MSH-related peptides in gastrin cells and suggest a heterogenous peptide make-up of endocrine cell granules.
