期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：9
页码：5421-5424
DOI：10.1073/pnas.78.9.5421
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Plasmids harboring the amino-terminal part of bacteriophage f1 gene II confer to bacterial cells partial resistance to infection with the male-specific bacteriophages f1 and f2. This effect (IP-2 phenotype) is due to the production of large amounts of an approximately 20,000-dalton polypeptide corresponding to the amino-terminal part of gene II protein. These results have allowed the isolation of clones producing functional gene II protein in large amounts. An in vitro assay has been developed to test the enzymatic activity of the gene II protein produced.
