期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：9
页码：5908-5912
DOI：10.1073/pnas.78.9.5908
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：It was found that digitonin-permeabilized resting gastric glands retained considerable acid-secretory ability. Oligomycin abolished this, and ATP was able to bypass this inhibition and restore acid secretion. Moreover, the effect of anoxia was also bypassed by ATP in these preparations. As in intact glands, acid secretion was K+ dependent, and the concentration for half-maximal effect was 18.5 +/- 1.76 mM in Na+-free solutions, a value similar to that found for resting intact glands. The slight inhibition of ATP-dependent secretion by either valinomycin or 2,4-dinitrophenol, but total inhibition by a combination of the ionophores, is interpreted to mean that, in resting gastric glands, the in situ proton pump is electroneutral and the KCl pathway supplying K+ to the luminal face of the pump is probably electroneutral.
