期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1986
卷号：83
期号：24
页码：9764-9767
DOI：10.1073/pnas.83.24.9764
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have established in vitro conditions under which we can reliably measure kinase activity in normal postmortem human brain. Using these conditions, we detected in the brains of patients with Alzheimer disease a 2-fold increase in the level of Mr 60,000 protein phosphorylation compared to age-matched controls. The Mr 60,000 protein phosphorylation was found exclusively in the cytosol fraction. No differences were detected between phosphoproteins in 100,000 X g pellet fractions from brains of Alzheimer disease patients and from age-matched controls. Postmortem time up to 17 hr does not seem to affect the phosphorylation level of the Mr 60,000 protein. Younger Alzheimer disease patients had more prominent changes in the elevation of the Mr 60,000 protein phosphorylation level than older patients, although in the control patient, age did not affect the phosphorylation level of the Mr 60,000 protein. We conclude that in the brain cytosol of Alzheimer disease there may be an abnormality in either the degree of Mr 60,000 protein phosphorylation or in the Mr 60,000 protein concentration.
