期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1991
卷号：88
期号：19
页码：8826-8830
DOI：10.1073/pnas.88.19.8826
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Neurospora VS RNA is a mitochondrial single-stranded RNA that combines certain features of catalytic RNAs and group I introns. We report here that monomeric VS RNA synthesized in vitro by self-cleavage of a multimeric transcript can perform an RNA-mediated self-ligation reaction producing circular RNAs indistinguishable from those isolated from mitochondria. We conclude that the active site for the ligation reaction is present in the RNA itself. Also, the mechanism for aligning the termini to be ligated may be different from mechanisms previously described. The lack of sequence similarity between VS RNA and previously characterized catalytic RNAs suggests that VS RNA is an independently evolved ribozyme capable of both cleavage and ligation.
