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  • 标题:Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies
  • 本地全文:下载
  • 作者:T Watanabe ; Y Ono ; Y Taniyama
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1992
  • 卷号:89
  • 期号:21
  • 页码:10159-10163
  • DOI:10.1073/pnas.89.21.10159
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.
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