标题：Intrathymic selection of NK1.1+α/β T cell antigen receptor (TCR)+ cells in transgenic mice bearing TCR specific for chicken ovalbumin and restricted to I-Ad
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：14
页码：8199-8204
DOI：10.1073/pnas.95.14.8199
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Generation and negative selection of NK1.1+/{beta} T cell receptor (TCR)+ thymocytes were analyzed using TCR-transgenic (B10.D2 x DO10)F1 and (C57BL/6 x DO10)F1 mice and Rag-1-/-/DO10 mice, which had been established by breeding and backcrossing between Rag-1-/- and DO10 mice. Almost all T cells from these mice were shown to bear V13/V{beta}8.2 that is specific for chicken ovalbumin (cOVA) and restricted to I-Ad. A normal proportion of the NK1.1+ V13/V{beta}8.2+ thymocytes was generated in these mice. However, the actual cell number of both NK1.1+ and NK1.1- thymocytes in I-Ad/d mice (positive selecting background) was larger than that in I-Ab/d mice (negative selecting background). Markedly low but significant proportions of NK1.1+ V13/V{beta}8.2+ cells were detected in the spleens from I-Ad/d and I-Ab/d mice. It was shown that the splenic NK1.1+ T cells of the I-Ab/d mice were anergized against stimulation through TCR. When (B10.D2 x DO10)F1 and (C57BL/6 x DO10)F1 mice were given cOVA, extensive or intermediate elimination of NK1.1+/{beta}TCR+ thymocytes was induced in I-Ad/d or I-Ab/d mice, respectively. However, the clonal elimination was not as complete as that seen in the major NK1.1- thymocyte population. The present findings indicate that normal generation of NK1.1+/{beta}TCR+ thymocytes occurs in the absence of V14-J281 and that substantial negative selection operates on the NK1.1+/{beta}TCR+ cells.
关键词：thymus/natural killer T cell/T cell antigen receptor transgenic mouse/negative selection
