期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：16
页码：9660-9664
DOI：10.1073/pnas.95.16.9660
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：To investigate the role of aquaporin-1 (AQP1) water channels in proximal tubule function, in vitro proximal tubule microperfusion and in vivo micropuncture measurements were done on AQP1 knockout mice. The knockout mice were generated by targeted gene disruption and found previously to be unable to concentrate their urine in response to water deprivation. Unanesthetized knockout mice consumed 2.8-fold more fluid than wild-type mice and had lower urine osmolality (505 {+/-} 40 vs. 1081 {+/-} 68 milliosmolar). Transepithelial osmotic water permeability (Pf) in isolated microperfused S2 segments of proximal tubule from AQP1 knockout [-/-] mice was 0.033 {+/-} 0.005 cm/s (SE, n = 6 mice, 37{degrees}C), much lower than that of 0.15 {+/-} 0.03 cm/s (n = 8) in tubules from wild-type [+/+] mice (P < 0.01). In the presence of isosmolar luminal perfusate and bath solutions, spontaneous fluid absorption rates (nl/min/mm tubule length) were 0.31 {+/-} 0.12 (-/-, n = 5) and 0.64 {+/-} 0.15 (+/+, n = 8). As determined by free-flow micropuncture, the ratios of tubular fluid-to-plasma concentrations of an impermeant marker TF/P in end proximal tubule fluid were 1.36 {+/-} 0.05 (-/-, n = 8 mice [53 tubules]) and 1.95 {+/-} 0.09 (+/+, n = 7 mice [40 tubules]) (P < 0.001), corresponding to 26 {+/-} 3% [-/-] and 48 {+/-} 2% [+/+] absorption of the filtered fluid load. In collections of distal tubule fluid, TF/P were 2.8 {+/-} 0.3 [-/-] and 4.4 {+/-} 0.5 [+/+], corresponding to 62 {+/-} 4% [-/-] and 76 {+/-} 3% [+/+] absorption (P < 0.02). These data indicate that AQP1 deletion in mice results in decreased transepithelial proximal tubule water permeability and defective fluid absorption. Thus, the high water permeability in proximal tubule of wild-type mice is primarily transcellular, mediated by AQP1 water channels, and required for efficient near-isosmolar fluid absorption.
