期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：26
页码：15598-15602
DOI：10.1073/pnas.95.26.15598
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have characterized the interaction between apolipoprotein E (apoE) and amyloid {beta} peptide (A{beta}) in the soluble fraction of the cerebral cortex of Alzheimer's disease (AD) and control subjects. Western blot analysis with specific antibodies identified in both groups a complex composed of the full-length apoE and A{beta} peptides ending at residues 40 and 42. The apoE-A{beta} soluble aggregate is less stable in AD brains than in controls, when treated with the anionic detergent SDS. The complex is present in significantly higher quantity in control than in AD brains, whereas in the insoluble fraction an inverse correlation has previously been reported. Moreover, in the AD subjects the A{beta} bound to apoE is more sensitive to protease digestion than is the unbound A{beta}. Taken together, our results indicate that in normal brains apoE efficiently binds and sequesters A{beta
