期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：13
页码：7451-7455
DOI：10.1073/pnas.96.13.7451
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We analyzed the cytolytic activity of intraepithelial T cells (IEL) isolated from the small intestines of 2- to 3-month-old mutant mice rendered deficient in different gene(s) in which the number of IEL expressing either T cell receptor (TCR)-{beta} ({beta}-IEL) or TCR-{gamma}{delta} ({gamma}{delta}-IEL) were absent or markedly diminished. When compared with wild-type littermates, cytolytic activity of {gamma}{delta}-IEL was sharply attenuated in TCR-{beta} mutant mice but remained unaltered in TCR- mutant mice in which a minor population of dull TCR-{beta}+ ({beta}dim)-IEL was also present. Cytolytic activity of {gamma}{delta}-IEL was maintained in mice doubly homozygous for {beta}2-microglobulin and transporter associated with antigen processing 1 gene mutations in which a conspicuous decrease was noted in absolute numbers of {beta}-IEL. In contrast, both TCR-{delta} and IL-7 receptor- gene mutations that lead to lack of {gamma}{delta}-IEL generation did not affect the development or cytolytic activity of the remaining {beta}-IEL. The anti-CD3 and anti-TCR-{gamma}{delta} mAb-induced IFN-{gamma} production of {gamma}{delta}-IEL showed the same TCR- and TCR-{beta} mutation-dependent variability. These results indicate that cytolytic and IFN-{gamma}-producing activities of {gamma}{delta} T cells in mouse intestinal epithelium are TCR-{beta}-chain-dependent.
