期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：13
页码：7575-7580
DOI：10.1073/pnas.96.13.7575
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Transgenic rice containing an antisense cDNA for the subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the -subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the subunit by antisense technology. Analysis of the cDNAs for the subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the -subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.
