期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：16
页码：9287-9292
DOI：10.1073/pnas.96.16.9287
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have identified expression of T-cell receptor {gamma} chain (TCR{gamma}) mRNA in human prostate and have shown that it originates from epithelial cells of the prostate and not from infiltrating T-lymphocytes. In contrast, the T-cell receptor {delta} chain (TCR{delta}) gene is silent in human prostate. The major TCR{gamma} transcript in prostate has a different size than the transcript expressed in thymus, spleen, and blood leukocytes. It is expressed in normal prostate epithelium, adenocarcinoma of the prostate, and the prostatic adenocarcinoma cell line LNCaP. The RNA originates from an unrearranged TCR{gamma} locus, and it is initiated within the intronic sequence directly upstream of the J{gamma}1.2 gene segment. The prostate-specific TCR{gamma} transcript consists of the J{gamma}1.2 and C{gamma}1 gene segments, and it has an untranslated sequence including a polyadenylation signal and poly(A) sequence at the 3'end. The finding that prostate epithelial cells express a high level of a transcript from a gene that was thought to by exclusively expressed by T-lymphocytes is highly unexpected.
