期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：18
页码：10385-10390
DOI：10.1073/pnas.96.18.10385
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Morphine and other {micro} opioids regulate a number of intracellular signaling pathways, including the one mediated by phospholipase C (PLC). By studying PLC {beta}3-deficient mice, we have established a strong link between PLC and {micro} opioid-mediated responses at both the behavioral and cellular levels. Mice lacking PLC {beta}3, when compared with the wild type, exhibited up to a 10-fold decrease in the ED50 value for morphine in producing antinociception. The reduced ED50 value was unlikely a result of changes in opioid receptor number or affinity because no differences were found in whole-brain Bmax and Kd values for {micro
