期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：2
页码：477-480
DOI：10.1073/pnas.96.2.477
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：An early folding event of cytochrome c populates a helix-containing intermediate (INC) because of a pH-dependent misligation between the heme iron and nonnative ligands in the unfolded state (U). For folding to proceed, the nonnative ligation error must first be corrected. It is not known whether I is on-pathway, with folding to the native state (N) as in U {leftrightarrow} INC {leftrightarrow} N, or whether the I must first move back through the U and then fold to the N through some alternative path (INC {leftrightarrow} U {leftrightarrow} N). By means of a kinetic test, it is shown here that the cytochrome c I does not first unfold to U. The method used provides an experimental criterion for rejecting the off-pathway I {leftrightarrow} U {leftrightarrow} N option.
