首页    期刊浏览 2024年11月24日 星期日
登录注册

文章基本信息

  • 标题:Optical detection of cytochrome P450 by sensitizer-linked substrates
  • 本地全文:下载
  • 作者:Ivan J. Dmochowski ; Brian R. Crane ; Jonathan J. Wilker
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1999
  • 卷号:96
  • 期号:23
  • 页码:12987-12990
  • DOI:10.1073/pnas.96.23.12987
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The ability to detect, characterize, and manipulate specific biomolecules in complex media is critical for understanding metabolic processes. Particularly important targets are oxygenases (cytochromes P450) involved in drug metabolism and many disease states, including liver and kidney dysfunction, neurological disorders, and cancer. We have found that Ru photosensitizers linked to P450 substrates specifically recognize submicromolar cytochrome P450cam in the presence of other heme proteins. In the P450:Ru-substrate conjugates, energy transfer to the heme dramatically accelerates the Ru-luminescence decay. The crystal structure of a P450cam:Ru-adamantyl complex reveals access to the active center via a channel whose depth (Ru-Fe distance is 21 A) is virtually the same as that extracted from an analysis of the energy-transfer kinetics. Suitably constructed libraries of sensitizer-linked substrates could be employed to probe the steric and electronic properties of buried active sites.
国家哲学社会科学文献中心版权所有