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  • 标题:Sorting by diffusion: An asymmetric obstacle course for continuous molecular separation
  • 本地全文:下载
  • 作者:Chia-Fu Chou ; Olgica Bakajin ; Stephen W. P. Turner
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1999
  • 卷号:96
  • 期号:24
  • 页码:13762-13765
  • DOI:10.1073/pnas.96.24.13762
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A separation technique employing a microfabricated sieve has been demonstrated by observing the motion of DNA molecules of different size. The sieve consists of a two-dimensional lattice of obstacles whose asymmetric disposition rectifies the Brownian motion of molecules driven through the device, causing them to follow paths that depend on their diffusion coefficient. A nominal 6% resolution by length of DNA molecules in the size range 15-30 kbp may be achieved in a 4-inch (10-cm) silicon wafer. The advantage of this method is that samples can be loaded and sorted continuously, in contrast to the batch mode commonly used in gel electrophoresis.
  • 关键词:DNA fractionation ; Brownian motion ; microfabricated array
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