期刊名称：Liminalities : a Journal of Performance Studies
电子版ISSN：1557-2935
出版年度：2014
卷号：58
语种：English
出版社：Liminalities
摘要：ABSTRACT:A study was conducted to determine the lethal dose of chemical mutagen EMS in rice cultivar ADT (R) 47. Genetically pure seeds were treated with different concentrations of EMS viz., 80mM, 100mM, 120mM, 140mM and 160mM. Untreated seeds soaked in distilled water was used as check for comparison. The LD50 values were observed based on growth reduction of seedlings after EMS treatment. The LD50 dose for EMS under invitro and invivo condition was fixed at 126mM and 121mM based on probit analysis. As the concentration of applied EMS increased, there was a decrease in germination, survival rate of seedlings, root length, shoot length, seedling height, vigour index under invitro conditions and emergence and survival under field (invivo) conditions in M1 generation as compared to the control. The survival percentage of seedlings was higher at higher doses of EMS under invivo conditions compared to invitro conditions.
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