摘要：In this country, market research have revealed that there has been adulteration in food products in which some plant species have been replaced with plant ingredients that have a lower economic value. Fraudulent practices involving pistachios, chestnuts and almonds are prominent among these activities. For the protection of consumers and to prevent unfair competition in the market, the detection of fraudulent practices is required with precise and reliable methods. In this study, a new method based on the real- time PCR TaqMan probe technique was developed to determine the fraudulent use of pea instead of pistachio, apricot kernel instead of almond and common bean instead of chestnut in some food products. For this purpose, species-specific primer and probe sets were designed on the chloroplast tRNA-Leu (trnL) gene for pea and bean and also on the nuclear S14 F- box protein gene for apricot. In the real-time PCR reactions performed with the designed species specific primer and probe sets, apricot could be detected at the level of 0.1%, pea at the level of 0.01% and bean at the level of 0.001%, quantitatively, without any cross-reactivity with other plant species examined. Additionally, the developed method also provided successful detection in binary mixtures of the plants: pistachio-pea, chestnut-bean and apricot kernel-almond. As a result, it was concluded that the method provided a sensitive and reliable detection method for pea, bean and apricot kernel residues in foods, and it can successfully be used in the routine control of fraudulent practices involving these plant species in food products.
