期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：39
页码：10408-10413
DOI：10.1073/pnas.1710496114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Diels–Alder reaction is one of the most common methods to chemically synthesize a six-membered carbocycle. While it has long been speculated that the cyclohexene moiety found in many secondary metabolites is also introduced via similar chemistry, the enzyme SpnF involved in the biosynthesis of the insecticide spinosyn A in Saccharopolyspora spinosa is the first enzyme for which catalysis of an intramolecular [ 4 + 2 ] -cycloaddition has been experimentally verified as its only known function. Since its discovery, a number of additional standalone [ 4 + 2 ] -cyclases have been reported as potential Diels–Alderases; however, whether their catalytic cycles involve a concerted or stepwise cyclization mechanism has not been addressed experimentally. Here, we report direct experimental interrogation of the reaction coordinate for the [ 4 + 2 ] -carbocyclase SpnF via the measurement of α -secondary deuterium kinetic isotope effects (KIEs) at all sites of s p 2 → s p 3 rehybridization for both the nonenzymatic and enzyme-catalyzed cyclization of the SpnF substrate. The measured KIEs for the nonenzymatic reaction are consistent with previous computational results implicating an intermediary state between formation of the first and second carbon–carbon bonds. The KIEs measured for the enzymatic reaction suggest a similar mechanism of cyclization within the enzyme active site; however, there is evidence that conformational restriction of the substrate may play a role in catalysis.
