期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:2017
卷号:114
期号:40
页码:E8488-E8497
DOI:10.1073/pnas.1706879114
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a eukaryotic mechanism that enables Hydra to specifically modify long-chain 3-oxo-homoserine lactones into their 3-hydroxy-HSL counterparts. Expression data revealed that Hydra ’s main bacterial colonizer, Curvibacter sp., responds differentially to N -(3-hydroxydodecanoyl)- l -homoserine lactone (3OHC12-HSL) and N -(3-oxododecanoyl)- l -homoserine lactone (3OC12-HSL). Investigating the impacts of the different N -acyl-HSLs on host colonization elucidated that 3OHC12-HSL allows and 3OC12-HSL represses host colonization of Curvibacter sp. These results show that an animal manipulates bacterial quorum-sensing signals and that this modification leads to a phenotypic switch in the bacterial colonizers. This mechanism may enable the host to manipulate the gene expression and thereby the behavior of its bacterial colonizers.
关键词:Hydra ; quorum quenching ; quorum sensing ; interference ; host–microbe interaction