标题：Biochemical Characteristics of Lactic Acid Bacteria with Proteolytic Activity and Capability as Starter Culture Isolated From Spontaneous Fermented Local Goat Milk
出版社：The International Institute for Science, Technology and Education (IISTE)
摘要：This study was aimed to isolate Lactic Acid Bacteria (LAB) from spontaneus fermented local goat milk, which had proteolytic activity and used for futher fermentation of goat milk. Isolation of LAB was carried out by MRS Agar and CaCo 3 . Identification species of isolates was conducted according to the Bergeys manual (conventional method) and based on morphological, physiological and biochemical analysis using API 50 CHL kit was also carried out. The results of identification and chemical test showed that 26 isolates were identified as LAB were 16 isolates (61.5%) with rod shape and 10 isolates (38.5%) with cocci shape. Based on proteolytic activity, the isolates composed of two bacteria species namely Lactobacillus plantarum (YN1.1, YN1.3, YN 1.8 and isolates YN 2.25). and the other bacteria was Lactobacillus pentosus (YN 1.6.). five isolates had proteolytic activity, where three of them had the best proteolytic activity namely Lactobacillus plantarum YN 1.1, Lactobacillus plantarum YN 1.3 and Lactobacillus pentosus YN 1.6 . All of these isolates had the ability to ferment goat milk which was indicated by the increase of bacteria viability reaching up to 10 9 cfu/ml and also decreaced of pH value at the final stage of fermentation. It can be concluded that isolated LAB from spontaneous fermented local goat milk could be used as starter culture and could be inproved as commercial product.
关键词：identification; API 50 CHL; Lactobacillus plantarum ; Lactobacillus pentosus ; viability; pH .
