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  • 标题:Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1
  • 其他标题:Production of Antibody Raised Against Lipopolysaccharide (LPS) of Vibrio cholerae Non-O1
  • 作者:M Saadati ; A Godarzi ; M Akbari
  • 期刊名称:Iranian Journal of Public Health
  • 印刷版ISSN:2251-6085
  • 电子版ISSN:2251-6093
  • 出版年度:2008
  • 卷号:37
  • 期号:2
  • 页码:15-23
  • 语种:English
  • 出版社:Tehran University of Medical Sciences
  • 摘要:Background: Cholera, an infectious disease caused by Vibrio cholerae, is primarily transmitted by ingestion of contaminated food or water. In severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death. Methods: In this study V.cholerae non-O1 was cultured in suitable media. LPS was extracted from the surface of bacteria by hot phenol-water method and then purified by high-speed centrifugation. For production of specific antibody against LPS, white newzeland rabbits were first immunized by whole cell bacteria and then immunized with highly purified LPS. The titre of the antiserum was determined by ELISA for each serogroup. Results: Results presented in this study indicate that serum anti-LPS antibodies raised against purified LPS of V.cholerae non-O1 can detect V.cholerae non-O1. Conclusion: This antibody had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa. So, this antibody can be used for for detection of V. cholerae non-O1.
  • 关键词:Immunoglobulin; Lipopolysaccharide; Vibrio cholerae non-O1; ELISA
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