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  • 标题:Solvent Optimization For Genistein Isolation Of “Rotten Tempe” By High Performance Liquid Chromatography Method
  • 本地全文:下载
  • 作者:Cucun Alep Riyanto ; Hartati Soetjipto
  • 期刊名称:Jurnal Eksakta
  • 印刷版ISSN:1411-1047
  • 电子版ISSN:2503-2364
  • 出版年度:2017
  • 卷号:17
  • 期号:2
  • 页码:111-118
  • DOI:10.20885/eksakta.vol17.iss2.art3
  • 语种:English
  • 出版社:Jurnal Eksakta
  • 其他摘要:Genistein is a soy isoflavone that has been known have anticancer properties. The aim of  this research is to determine the right combination of solvents to extract isoflavones in rotten tempe  and determine the genistein content of the extraction process by the High Performance Liquid  Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of  methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High  Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio.  Where as the highest contents of genistein from the 4 th day “rotten tempe” as 26.199 ± 25.146 (mg/g).
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