The growth of scientific attention to the problem of culture functioning at all levels is due to several factors: scientific interest in gumanistica; comprehension of culture as a phenomenon that penetrates all social spheres; scientific integrative processes strengthening; the actualization of humanitarian thinking. Article aims to develop the content, ways and pedagogical means of future teacher professional culture formation. Our research of this process is based on the culturological approach that is one of the modern methodological approaches in pedagogy. The results of the theoretical and experimental research work in the field of teacher professional culture formation are presented. It is needed to rely on a stating that the professional competence of a teacher is not the only goal-result of higher professional education. Modern school education also needs a teacher of high professional culture. In the structure of the teacher professional culture there are three components: evaluative, active and creative. Their formation requires educational training process reconstruction on the principles of culturological approach. Pedagogical experiment has confirmed the effect of proposed conditions and means of teacher professional culture formation The problem revealed in the article, may be useful for higher school teachers; researchers in the pedagogical problem of education and culture integration; students of pedagogical skills.