Four new lignans, a furofuran lignan medioresinol B ( 10 ) and three tetrahydrofuran lignans kobusinol C ( 16 ), 7′-methoxy magnostellin A ( 21 ), and mangnostellin D ( 23 ), along with 19 known lignans, were isolated from the flower buds of Magnolia biondii PAMP. The structures of the isolates were elucidated using spectroscopic analysis, mainly one- and two-dimensional NMR, high resolution-MS, and circular dichroism techniques as well as Mosher’s esterification method. The anti-allergic effects of the isolated compounds were evaluated by analyzing the inhibition of interleukin-2 (IL-2) expression in Jurkat T-cells. Compounds 11 – 14 reduced IL-2 expression in a dose-dependent manner.