The purpose of this study was to investigate the effects of sprint training (30-s maximal cycle ergometer sprinting) on bicarbonate buffering capacity and anaerobic capacity. Fourteen healthy males were assigned to either a training group (TG; n=7) or a control group (CG; n=7). The TG performed sprint training 2 days per week for 8 weeks. Before and after training, exercise performance (mean power) and excess CO_2 output (ExcessCO_2) were measured by 30-s maximal cycle ergometer sprinting, and maximal accumulated oxygen deficit (MAOD) was measured by 2-3 min exhaustive cycling test. Mean power (pre: 8.5±0.9W/kg, post: 9.3±0.8W/kg, p<0.01), ExcessCO_2 (pre: 93.4±12.8ml/kg, post: 102.3±12.2ml/kg, p<0.05), and MAOD (pre: 59.6±14.4ml/kg, post: 68.2±11.0ml/kg, p<0.01) were significantly increased after training in the TG. A significant correlation was demonstrated between percentage change in ExcessCO_2 and that in MAOD (r=0.755, p<0.05). Before and after training, mean power was significantly correlated with ExcessCO_2 and MAOD, respectively. These results suggest that the increase in ExcessCO_2 caused by sprint training may enhance the energy supplied from anaerobic metabolism, and improve short-duration intensive exercise performance.