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  • 标题:The Effects of ZnO Nanoparticles in Combination with Alcohol on Biosynthetic Potential of Saccharomyces cerevisiae
  • 本地全文:下载
  • 作者:Natalia Chiseliţa ; Agafia Usatii ; Nadejda Efremova
  • 期刊名称:Acta Universitatis Cibiniensis. Series E: Food Technology
  • 印刷版ISSN:2344-150X
  • 出版年度:2017
  • 卷号:21
  • 期号:2
  • 页码:19-24
  • DOI:10.1515/aucft-2017-0011
  • 语种:English
  • 出版社:Walter de Gruyter GmbH
  • 摘要:This paper reports about experimental results concerning the influence of 30 nm ZnO nanoparticles on biomass, carbohydrates, β-glucans, proteins accumulation and catalase enzyme activity at Saccharomyces cerevisiae CNMN-Y-20 yeast strain exposed to alcohol action. Alcohol in concentrations of 2%, 5% and 10% added to culture medium has been reported to stimulate β-glucans biosynthesis and to inhibit protein synthesis. Low biomass production, with 71% less that control, was detected in the experiments with 10% alcohol. ZnO nanoparticles in combination with alcohol do not offer sufficient protection for the proteins biosynthesis, but efficiently protect the carbohydrates and β-glucans biosynthetic processes, which contents in the biomass are with 16.6% and 19.9% higher than control, respectively. The maximum value of β-glucans content was established in case of cultivation of selected yeast strain on YPD medium supplemented with 5 mg/L nanoparticles ZnO and 2% alcohol. The obtained results allowed the elaboration of new procedure for directed synthesis of β-glucans that contributed to an increase of this component with 30.7%, compared to control.
  • 关键词:Saccharomyces cerevisiae ; nanoparticles ; alcohol ; biomass production ; carbohydrates ; β-glucans ; proteins ; catalase activity
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